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A variety of products designed for use in various PCR procedures. Includes dNTPs, enzymes, assays, arrays, master mixes, reagents, and kits. Products can be used for standard, real-time, direct, high fidelity, hot start, and long range PCR protocols.
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LunaScript RT Master Mix Kit (Primer-free) is an optimized master mix containing all the necessary components for first strand cDNA synthesis except for primers. The mix is compatible with random primers, oligo dT primers, and gene-specific primers enabling maximum cDNA synthesis flexibility. LunaScript RT Master Mix (Primer-free) features the thermostable Luna Reverse Transcriptase which supports cDNA synthesis at elevated temperatures. Murine RNase Inhibitor is included to protect template RNA from degradation. In addition, the presence of a blue tracking dye provides a visual indicator for the RT step as well as downstream applications.
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GenScript M-MuLV Reverse Transcriptase (M-MLV) is derived from a cloned region of the pol gene of MMLV and isolated from an E coli strain overexpressing this construct To increase cDNA yields and get a higher percentage of longer transcripts the M-MLV Reverse Transcriptase has been modified with reduced RNase H activity and expressed free of exogenous RNases and other nucleases The enzyme can synthesize a complementary cDNA strand initiating from a primer using RNA as template (cDNA synthesis) making it ideal for a wide range of applications
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10 mM dNTP Mix is suitable for use in PCR sequencing nick translation fill-ins cDNA synthesis TdT tailing reactions and the dilution of radiolabeled dNTPs These nucleotides are supplied as convenient ready-to-use solutions in sterilized water The 10 mM dNTP Mix contains all four dNTPs each at a final concentration of 10 mM
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GenScript High-Stability PCR Kit contains Green i Taq /i DNA polymerase 10 Taq buffer and Stabilized dNTP Mix 10 mM Each all specially formulated for increased stability using our proprietary technology The kit can be stored at 4 for over six months or at room temperature (25 ) for one month without significant loss of activity The stabilizers in the buffers also increase the stability of Green Taq DNA polymerase at higher temperature (72 ) increasing the DNA yield of PCR amplifications of long DNA This kit facilitates quick and easy PCR setup without any need for thawing This kit can also be used in high-throughput assays in which all the PCR reagents need to be ready and stable for PCR setup at any time
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Hot Start Taq DNA Polymerase is a recombinant thermostable Taq DNA polymerase complexed with a thermolabile neutralizing antibody that blocks the polymerase activity prior to the initial DNA denaturation step of PCR When the temperature of the PCR reaction mix reaches 95 C during the initial DNA denaturing step of PCR cycling activity of the Taq DNA polymerase is fully restored br/
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Suitable for use in PCR sequencing nick translation fill-in cDNA synthesis TdT tailing reactions and dilution of radiolabeled dNTPs These nucleotides are supplied as convenient ready-to-use solutions in Tris-HCl (pH 7 5) The 10 mM HS dNTP Mix contains all four dNTPs each at a final concentration of 10 mM It also contains a stabilizer which makes it a very long-lasting tool
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GoldBio s 2 -Deoxynucleoside 5 -triphosphate mix is an aqueous solution containing dATP dCTP dGTP and dTTP each at a final concentration of 10mM This mixture has been tested for performance in a wide size-range of PCR templates with Taq and Pfu DNA polymerases
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